Summary
Type: TLC
Application #: 400871
Catalog#: 2614156 - C18 Silica HPTLC Plates, w/UV254, 50% silanization, glass backed, 200um, 10x10cm, 25/pk
Detection: UV 254 nm
Conditions: Eluents Method 1: 2.5 % MgSO4 in water / methanol (90:10)
Eluents Method 2: AcOH / CH3OH / water (10:20:70)
Eluents Method 3: 0.5 % KBr in water / methanol (80:20)
Eluents Method 4: 1 % KBr in water / methanol (80:20)
Migration Distance: 6 cm
Sample volume: 0.3 - 0.5 μm of a sample dissolved in water / methanol (1:1)
Authors: Lepri L Coas V Desideri P G Pettini L Checcini L
Source: J Planar Chromatogr 2 (1989) 456-460
Details
Legend: Nucleotide
hRf value with method
1
2
3
4
2',3'-cAMP
45
44
34
38
3',5'-cAMP
36
42
25
30
d-3',5'-cAMP
39
42
30
34
2',3'-cGMP
71
22
39
45
3',5'-cGMP
62
18
28
35
d-3